Prediction of in vivo bone forming potency of bone marrow-derived human mesenchymal stem cells
1 Research Centre for Experimental Orthopaedics, Orthopaedic University Hospital Heidelberg, Heidelberg, Germany
§Current address: Department of Orthopaedic Surgery, University of Dresden, Dresden, Germany
Abstract
Human mesenchymal stem cells (MSC) have attracted much attention for tissue regeneration including repair of non-healing bone defects. Heterogeneity of MSC cultures and considerable donor variability however, still preclude standardised production of MSC and point on functional deficits for some human MSC populations. We aimed to identify functional correlates of donor-dependency of bone formation in order to develop a potency assay predicting the therapeutic capacity of human MSC before clinical transplantation. MSC from 29 donors were characterisedin vitroand results were correlated to bone formation potency in a beta-tricalcium-phosphate (β-TCP)-scaffold after subcutaneous implantation into immunocompromised mice.
Keywords
- Bone tissue engineering
- beta-tricalcium-phosphate
- heterotopic bone formation
- mesenchymal stem cell
- cell proliferation
- prediction
- microarray
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