Stimulatory effects of creatine on metabolic activity, differentiation and mineralization of primary osteoblast-like cells in monolayer and micromass cell cultures
I. Gerber 1*
, I. ap Gwynn 2, M. Alini 3, T. Wallimann 1
, I. ap Gwynn 2, M. Alini 3, T. Wallimann 1Affiliations
Article Info
1 Institute of Cell Biology, ETH Hoenggerberg, CH-8093 Zurich, Switzerland
2 Biological Sciences, The University of Wales, Aberystwyth, Ceredigion SY23 3DA, Wales, U.K.
3 AO Research Institute, Clavadelerstrasse, CH-7270 Davos Platz, Switzerland
Abstract
The effects of creatine (Cr) supplementation on primary rat osteoblast-like cells cultured as monolayer and micromass were investigated. Cr was added to the medium at concentrations of either 10 or 20 mM. At various time points, the cell cultures were analyzed morphologically, metabolically and biochemically.
Keywords
- Osteoblast-like cells
- creatine
- viability
- metabolic activity
- ultrastructure
- differentiation
- mineralization
- monolayer culture
- micromass culture
- cell protection
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