Mineralization of regenerated cellulose hydrogels induced by human bone marrow stromal cells
, B. De Jéso 3, R. Bareille 4, F. Rouais 4, C. Baquey 4, M. A. Barbosa 1,21 INEB - Instituto de Engenharia Biomédica, Laboratório de Biomateriais, Rua do Campo Alegre, 823 - 4150-180 Porto, Portugal
2 Universidade do Porto, Faculdade de Engenharia, Dept. Engenharia Metalúrgica e de Materiais
3 Institut du Pin, Laboratoire de Chimie des Substances Végétales (LCSV), Université Bordeaux 1, Cours de la Libération - 33405 Talence Cedex, France
4 INSERM U.443, Université Bordeaux 2, 146 R. Léo Saignat, Bâtiment 4a - 33076 Bordeaux, France
Abstract
The proliferation of cultured human bone marrow stromal cells (HBMSC) on regenerated cellulose hydrogels was assessed. Regenerated cellulose hydrogels showed good rates of HBMSC proliferation, the cells exhibiting a flattened morphology, and after 22 days in culture, the cells had homogeneously colonized the surface of the materials. Moreover, since the early days in culture, between the surface of the materials and attached cells a continuous granulated hydroxyapatite layer was formed. It has been previously demonstrated in vitro, but without cells, that these materials did not mineralize. Hence, it seems that HBMSC promoted the mineralization of the surface.
Keywords
- Regenerated cellulose hydrogels
- human bone marrow stromal cells
- cell proliferation
- mineralization
- calcium phosphates
- hydroxyapatite
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