Cell volume increase in murine MC3T3-E1 pre-osteoblasts attaching onto biocompatible Tantalum observed by magnetic AC mode Atomic Force Microscopy
, S Antoranz Contera 1, J Justesen 2, M Duch 2, O Hansen 3, J Chevallier 1, M Foss 1, FS Pedersen 2, F Besenbacher 1*1 Interdisciplinary Nanoscience Center (iNANO) and Department of Physics and Astronomy, University of Aarhus, DK-8000 Aarhus, Denmark
2 Interdisciplinary Nanoscience Center (iNANO) and Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark
3 MIC - Department of Micro and Nanotechnology, Technical University of Denmark, DK-2800 Lyngby, Denmark
* These authors contributed equally as corresponding authors
Abstract
Magnetic AC mode (MACmode) atomic force microscopy (AFM) was used to study murine (mouse) MC3T3-E1 preosteoblastic cells attached to biocompatible tantalum substrates. Cell volumes of attached cells derived from AFM images were compared to volumes of detached cells in suspension measured by the Coulter sizing technique. Anincrease of ~ 50 % in cell volume was observed when the cells attached to planar tantalum substrates and developed a flattened structure including lamellipodia. We address thoroughly the issues general to the AFM determination of absolute cell volumes, and compare our magnetic AC mode AFM measurements to hitherto reported cell volumedeterminations by contact mode AFM.
Keywords
- AFM
- MACmode
- Cell volume
- Osteoblast
- Bone
- Biocompatibility
- Implant
- Tantalum
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